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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 79 - 84 (6page)
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Objective : Nypa fruticans Wurmb. Fruit (NF) has been used as a conventional medicine to treat inflammatory peridontal diseases in Myanmar and Eastern Asia. However, the anti-inflammatory effect of NF aqueous extract on lipopolysaccharide (LPS)-induced inflammatory responses was not well-investigated. Therefore, this study was aimed to investigate the anti-inflammatory effect of NF on LPS-induced inflammatory responses on RAW 264.7 cells.Methods : To induce inflammation on the macrophage cell line, RAW 264.7 cells were treated with 500 ng/mL of LPS. Water extracts of NF was treated 1 h prior to treatment of LPS. Cell viability was measured by MTT assay. Production of nitrite was measured with Griess assay and pro-inflammatory cytokines such as interleukine (IL)-1β and IL-6, and tumor necrosis factor (TNF)-α was measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR). In addition, we examined the inhibitory mechanisms of NF by western blot and immunocytochemistry.Result : Water Extract from NF itself did not have any cytotoxic effect at the concentration of 200 ㎍/ml in RAW 264.7 cells. Treatment of NF inhibited the production of nitrite, and pro-inflammatory cytokines inlcuding IL-1β, IL-6 and TNF-α in a dose dependant. In addition, NF treatment inhibited the LPS-induced activation and translocation of nuclear factor (NF)-κB.Conclusion : In summary, our result suggest that treatment of NF could reduce the LPS-induced inflammatory responses via deactivation of NF-κB. This study could suggest that NF could be a beneficial drug or agent to prevent inflammation.
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참고문헌 (18)
참고문헌 신청
McDaniel ML / 1996 / Cytokines and nitric oxide in islet inflammation and diabetes / Proc Soc Exp Biol Med 211:24~32
김대희 / 2009 / LPS로 활성화된 대식세포에서 黃連解毒湯 물추출물의 염증매개물질 억제효과 / 대한본초학회지 24(4):39~47
Behrens EM / 2008 / Macrophage activation syndrome in rheumatic disease: What is the role of the antigen presenting cell? / Autoimmunity Reviews 305:305~308
Lopez-Bojorquez LN / 2004 / Molecular mechanisms involved in the pathogenesis of septic shock / Arch Med Res 35:465~479
Athman R / 2004 / Innate immunity via Toll-like receptors and Nod proteins / Curr Opin Microbiol 7:25~32
김대희 / 2009 / LPS로 활성화된 대식세포에서 黃連解毒湯 물추출물의 염증매개물질 억제효과 / 대한본초학회지 24(4):39~47
Behrens EM / 2008 / Macrophage activation syndrome in rheumatic disease: What is the role of the antigen presenting cell? / Autoimmunity Reviews 305:305~308
Lopez-Bojorquez LN / 2004 / Molecular mechanisms involved in the pathogenesis of septic shock / Arch Med Res 35:465~479
Athman R / 2004 / Innate immunity via Toll-like receptors and Nod proteins / Curr Opin Microbiol 7:25~32
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