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논문 기본 정보

자료유형
학술저널
저자정보
Li Yuchan (School of Life Science and Engineering Southwest University of Science and Technology) Zhao Jun (School of Life Science and Engineering Southwest University of Science and Technology) Chen Hua (School of Life Science and Engineering Southwest University of Science and Technology) Yu Xia (Yongchuan Hospital of Chongqing Medical University) Li Hui (School of Life Science and Engineering Southwest University of Science and Technology) Zhang Yu (School of Life Science and Engineering Southwest University of Science and Technology) Feng Liang (School of Life Science and Engineering Southwest University of Science and Technology) Wu Zhe (School of Life Science and Engineering Southwest University of Science and Technology) Xie Wenlin (School of Life Science and Engineering Southwest University of Science and Technology) Hou Dabin (School of Life Science and Engineering Southwest University of Science and Technology) Yu Ma (School of Life Science and Engineering Southwest University of Science and Technology)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제17권 제2호
발행연도
2023.4
수록면
159 - 169 (11page)
DOI
10.1007/s11816-022-00772-y

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Bupleurum chinense DC is an important medicinal plant with many active ingredients that are used for the treatment of dif- ferent types of diseases and valued in pharmaceutical markets. In vitro shoot regeneration can efficiently contribute to the improvement of B. chinense. In the present study, we investigated the effects of the explant type and plant growth regulators (PGRs) on embryogenic callus induction and plant regeneration in B. chinense. Our investigation demonstrated that 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 1 mg/L thidiazuron (TDZ) played a major role in promoting callus induction from leaf, hypocotyl and stem 2 explants, whereas the most effective treatment for stem 1 callus formation was Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L 6-benzyladenine (BA) and 0.5 mg/L kinetin (Kin). The highest shoot regeneration rate (57.14%) was obtained from hypocotyl-induced calli in MS medium with 0.5 mg/L Kin after 12 weeks of cultivation. This regeneration protocol can be used in large-scale cultivation and may be useful for future genetic modifications of B. chinense.

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